Author(s): Xuan L, Tanaka H, Xu Y, Shoyama Y
Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG2a and IgG2b possessing lambda light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/19003338
Author(s): Pharmacopeia of the People’s Republic of China, Pharmacopoeia Commission of People\'s Republic of China, 2010.
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