Preparative isolation and purification of hydroxyl anthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution

Author(s): Yang F, Zhang T, Tian G, Cao H, Liu Q, Ito Y


Analytical and preparative high-speed counter-current chromatography was successfully used for the isolation and purification of hydroxyanthraquinones from Rheum officinale Baill (Dahuang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion and aloe-emodin were isolated each at over 98% purity.

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