Protective effect of  Aframomummelegueta phenolics against CCl(4)-induced rat hepatocytes damage; role of apoptosis and pro-inflammatory cytokines inhibition

Author(s): El-Halawany AM, El Dine RS, El Sayed NS, Hattori M


Aframomum melegueta is a commonly used African spice. Through a hepatoprotective bioassay-guided isolation, the chloroform fraction of A.melegueta seeds yielded one new diarylheptanoid named 3-(S)-acetyl-1-(4′-hydroxy-3′, 5′-di methoxyphenyl)-7-(3″,4″, 5″-trihydroxyphenyl)heptane (1) and two new hydroxyphenylalkanones, [8]-dehydrogingerdione (2) and [6]-dehydroparadol (3), in addition to six known compounds (4–9). The hepatoprotective effect of A. melegueta methanol extract, sub-fractions and isolated compounds was investigated using carbon tetrachloride (CCl4)-induced liver injury in a rat hepatocytes model. The methanol, chloroform extracts and compounds 1, 5, 8 and 9 of A. melegueta significantly inhibited the elevated serum alanine aminotransferase (ALT), thiobarbituric acid reactive substances (TBARS), tumor necrosis factor (TNFα), interleukin-1beta (Il-1β), caspase3 and 9 and enhanced the reduced liver glutathione (GSH) level caused by CCl4 intoxication. These results indicate that A.melegueta extracts and isolated compounds play a protective role in CCl4 induced acute liver injury which might be due to elevated antioxidative defense potentials, suppressed inflammatory responses and apoptosis of liver tissue.

Similar Articles

Natural products as sources of new drugs over the period 1981-2002

Author(s): Newman DJ, Cragg GM, Snader KM

Antimicrobial activity of some medicinal plants from Ghana

Author(s): Konning GH, Agyare C, Ennison B

In vitro bactericidal activity of diterpenoids isolated from Aframomum melegueta K

Author(s): Ngwoke KG, Chevallier O, Wirkom VK, Stevenson P, Elliott CT, et al.

Mild traumatic brain injury in U

Author(s): Hoge CW, McGurk D, Thomas JL, Cox AL, Engel CC, et al.

Injured Fluoro-Jade-positive hippocampal neurons contain high levels of zinc after traumatic brain injury

Author(s): Hellmich HL, Eidson KA, Capra BA, Garcia JM, Boone DR, et al.

Clinical trials in traumatic brain injury: lessons for the future

Author(s): Doppenberg EM, Choi SC, Bullock R

Emerging treatments for traumatic brain injury

Author(s): Xiong Y, Mahmood A, Chopp M