Author(s): Roy M, Sinha D, Mukherjee S, Paul S, Bhattacharya RK
Chronic arsenic exposure causes skin diseases, gastrointestinal and neurological disorders, diabetes and cancer in various organs. Oxidative stress associated with arsenic exposure cause genetic instabilities and may initiate carcinogenesis. Phytochemicals present in vegetables, fruits, spices, tea, and medicinal plants, have shown to suppress experimental carcinogenesis in various organs. The aim of the present study was to elucidate the protective effect of some of the phytochemicals against the arsenite induced DNA damage in normal mammalian V79 cells. Comet assay was used for assessment of DNA damage and 2', 7'-dichlorofluorescein dihydroacetate for estimation of ROS generated by arsenite. The effect of the phytochemicals was observed during simultaneous treatment with arsenic, before arsenite exposure and during repair experiments. Of all the phytochemicals tested against arsenic, curcumin gave better protection during simultaneous treatment and resveratrol during pre treatment, which was evident both from comet assay and ROS generation experiments. During pre treatment a longer duration of treatment with lower dose of phytochemicals proved fruitful in reducing the genotoxicity. During repair experiments the phytochemicals enhanced recovery of DNA damage and ellagic acid gave promising results. The results indicated that natural phytochemicals may have the efficacy in reducing arsenic induced genotoxicity, in scavenging ROS and in enhancing the process of DNA repair in V79 cells.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/19024166
Author(s): Adomi OP, Umukoro GE
Author(s): Ebiloma GU, Omale J, Aminu RO
Author(s): Adesida GA, Adesogan EK
Author(s): Prohp TP, Onoagbe IO
Author(s): Lorke D
Author(s): Gonzalez-Avila MM, Arriaga-Alba M, Garza de la, Carmen HP, del Dominguez-Ortiz M, et al
Author(s): Singh BN, Singh BR, Singh RL, Prakash D, Dhakarey R, et al.
Author(s): Vuillaume M
Author(s): Witz G
Author(s): Trush MA, Kensler TW
Author(s): Jang D, Murrell GA
Author(s): Passos DC, Ferreira HD, Vieira ILFB, Nunes WB, Felício LP
Author(s): Wan-Ibrahim WI, Sidik KS, Kuppusam UR
Author(s): Serpeloni JM, Bisarro dos Reis M, Rodrigues J, Campaner dos Santos L, Vilegas W, et al.
Author(s): Burrows CJ, Muller JG
Author(s): Lee JC, Kim HR, Kim J, Jang YS
Author(s): Halliwell B, Gutteridge JM