Proteome-level investigation of Brassica carinata-derived resistance to Leptosphaeriamaculans

Author(s): Subramanian B, Bansal VK, Kav NN


Plants resistant to the fungal pathogen Leptosphaeria maculans were generated by an interspecific cross between the highly susceptible Brassica napus (canola) and the highly resistant Brassica carinata. Changes in the leaf protein profiles of these lines were investigated in order to understand the biochemical basis for the observed resistance. Two-dimensional electrophoresis followed by tandem mass spectrometry led to the identification of proteins unique to the susceptible (5 proteins) and resistant genotypes (7 proteins) as well those that were differentially expressed in the resistant genotype 48 h after challenge with the pathogen (28 proteins). Proteins identified as being unique in the resistant plant material included superoxide dismutase, nitrate reductase, and carbonic anhydrase. Photosynthetic enzymes (fructose bisphosphate aldolase, triose phosphate isomerase, sedoheptulose bisphosphatase), dehydroascorbate reductase, peroxiredoxin, malate dehydrogenase, glutamine synthetase, N-glyceraldehyde-2-phosphotransferase, and peptidyl-prolyl cis-trans isomerase were observed to be elevated in the resistant genotype upon pathogen challenge. Increased levels of the antioxidant enzyme superoxide dismutase were further validated and supported by spectrophotometric and in-gel activity assays. Other proteins identified in this study such as nitrate reductase and peptidylprolyl isomerase have not been previously described in this plant-pathogen system, and their potential involvement in an incompatible interaction is discussed.

Similar Articles

Potential anti-cancer activities of Furanodiene, a Sesquiterpene from Curcuma wenyujin

Author(s): Sun XY, Zheng YP, Lin DH, Zhang H, Zhao F, et al.

Zerumbone, a tropical ginger sesquiterpene, inhibits colon and lung carcinogenesis in mice

Author(s): Kim M, Miyamoto S, Yasui Y, Oyama T, Murakami A, et al.

Anti-inflammatory sesquiterpenes from Curcuma zedoaria

Author(s): Makabe H, Maru N, Kuwabara A, Kamo T, Hirota M

Antimicrobial activity and cytotoxicity of the essential oil of Curcuma zedoaria

Author(s): Lai EY, Chyau CC, Mau JL, Chen CC, Lai YJ, et al.

Lectins, lectin genes, and their role in plant defense

Author(s): Chrispeels MJ, Raikhel NV

A proteomic analysis of Curcuma comosaRoxb

Author(s): Boonmee A, Srisomsap C, Chokchaichamnankit D, Karnchanatat A, Sangvanich P