Quantitative determination of pectic substances as an example of a rhamnopolysaccharide assay

Author(s): Turecek PL, Buxbaum E, Pittner F


A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose dehydrogenase under concomitant reduction of NAD, thus allowing the quantitative evaluation of the original pectin.

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