Author(s): Mosmann T
A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision. No washing steps are used in the assay. The main adavantages of the colorimetric assay are its rapidly and precision, and the lack of any radioisotope. We have used the assay to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Author(s): American Cancer Society
Author(s): WHO
Author(s): Kune GA, Vitetta L
Author(s): Watson AJ, Collins PD
Author(s): Sachse C, Smith G, Wilkie MJ, Barrett JH, Waxman R, Sullivan F, Forman D, Bishop DT, Wolf CR
Author(s): Shakirin FH, Prasad KN, Ismail A, Yuon LC, Azlan A
Author(s): Shakirin FH, Azlan A, Ismail A, Amom Z, Yuon LC
Author(s): Zhang L-l, Lin Y-M
Author(s): Basri DF, Fan SH (2005)
Author(s): Birasuren B, Kim NY, Jeon HL, Kim MR
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