Author(s): Weingarten H, Feder J
Collagenase from normal human skin fibroblasts was found to catalyze the hydrolysis of esters and thio esters. This observation led to the development of a rapid, sensitive, continuous spectrophotometric assay for vertebrate collagenase using the thio peptolide Ac-ProLeuGly-S-LeuLeuGly-OC2H5 as substrate in the presence of 4,4'-dithiodipyridine or Ellman's Reagent. A Km of 0.004 M and a kcat of 370,000 h-1 were determined for the thio peptolide-enzyme reaction. The method is able to detect collagenase at concentrations as low as 2 ng/ml.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/2990254
Author(s): ICCO
Author(s): Azhar I, Lee, MT
Author(s): Azizah O, Amin I, Nawalyah AG, Ilham A
Author(s): Kim A, Keeny PG
Author(s): María Elena CMF, Soengas P, Velasco P, et al.
Author(s): Perona JS, Cabello-Moruno R, Ruiz-Gutierrez V
Author(s): Halliwell B, Gutteridge JMC
Author(s): Allaith AAA
Author(s): Amin I, Zamaliah MM, Chin WF
Author(s): Dreosti IE
Author(s): Gasser P, Lati E, Peno-Mazzarino L, Bouzoud D, Allegaert L, et al.
Author(s): Young HK, Chan BC, Jin GK, Kang IK, Sun HP, et al.
Author(s): Yang HH, Eun YJ, Dong ON, Hyung JS
Author(s): Hammerstone JF, Lazarus SA, Mitchell AE, Rucker R, Schmitz HH
Author(s): Singleton VL, Rossi JA
Author(s): Benzie IF, Strain JJ
Author(s): Prasad NK, Divakar S, Shivamurthy GR, Aradhya SM
Author(s): Meda A, Lamien CE, Romito M, MillogoJ, Nacoulma OG
Author(s): Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, et al.
Author(s): Nelson BC, Sharpless KE
Author(s): Zhu QY, Hammerstone JF, Lazarus SA, Schmitz HH, Keen CL
Author(s): Misnawi,Jinap S, Nazamid S, Jamilah B
Author(s): Nazaruddin R, Seng LK, Hassan O, Said M
Author(s): Jinap S, Dimick PS,Hollender R
Author(s): Azizah O, Abbe MMJ, Kong, KW, Amin I, Nawalyah AG, Ilham A
Author(s): Demina NS, Lysenko SV
Author(s): Kim Y, UyamaH, Kobayashi S
Author(s): Miao YP, Wen R, Aoshima H, Zhou PG
Author(s): An BJ, Kwak JH, Park JM, Lee JY, Park TS, et al.
Author(s): BaylacS, Racine P
Author(s): Madhan B, Krishnamoorthy G, Rao JR, Nair BU
Author(s): Thring TS, Hili P, Naughton DP
Author(s): Meyer W, Neurand K, Radke B
Author(s): Gil MI, Tomas-Barberan FA, Hess-Pierce B, Kader AA
Author(s): Sun T, Ho CT
Author(s): Gorinstein S, Lojek A, Ciz M, Pawelzik E, Delgado-Licon E, et al.
Author(s): Gardner PT, White TAC, McPhail DB, Duthie GG
