Author(s): Lam HW, Lin HC, Lao SC, Gao JL, Hong SJ, et al.
Angiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JNK 1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/17497682
Author(s): Cheung LW, Leung KW, Wong CK, Wong RN, Wong AS
Author(s): Fan TP, Yeh JC, Leung KW, Yue PY, Wong RN
Author(s): Majewska I, Gendaszewska-Darmach E
Author(s): Tang JY, Li S, Li ZH, Zhang ZJ, Hu G, et al.
Author(s): Hong SJ, Wan JB, Zhang Y, Hu G, Lin HC, et al.
Author(s): Zhang Y, Hu G, Lin HC, Hong SJ, Deng YH, et al.
Author(s): Chung BH, Cho YL, Kim JD, Jo HS, Won MH, et al.
Author(s): Lee TH, Lee GW, Kim CW, Bang MH, Baek NI, et al.
Author(s): Meng H, Guo J, Sun JY, Pei JM, Wang YM, et al.
Author(s): Tam JC, Lau KM, Liu CL, To MH, Kwok HF, et al.
Author(s): Cárdenas C, Quesada AR, Medina MA
Author(s): Zheng KY, Choi RC, Cheung AW, Guo AJ, Bi CW, et al.
Author(s): Lin CM, Chiu JH, Wu IH, Wang BW, Pan CM, et al.
Author(s): Lee JH, Kim HL, Lee MH, You KE, Kwon BJ, et al.
Author(s): Liao QF, Bi KS
Author(s): Hu G, Long C, Murrell G, Liu K
Author(s): Hwang B, Cho J, Hwang IS, Jin HG, Woo ER, et al.
Author(s): Süntar IP, Akkol EK, Yalçin FN, Koca U, Keles H, et al.
Author(s): Chiou WF, Chen CC, Hung MH
Author(s): Lin CN, Tome WP
Author(s): Shokrzadeh M, SaeediSarvari SS
Author(s): Lin CN, Tome WP
Author(s): Potente M, Gerhardt H, Carmeliet P
Author(s): Singer AJ, Clark RA
Author(s): Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, et al.