Author(s): Ruijters EJ, Weseler AR, Kicken C, Haenen GR, Bast A
Accumulating evidence suggests that foods rich in flavanols decrease the risk of developing cardiovascular diseases. Attenuation of oxidative stress was suggested to contribute to the cardiovascular benefit of flavanols. Up to now it was unclear whether flavanol metabolites can also protect cells from oxidative stress. The aim of the present study was to determine the potential contribution of several glucuronidated, methylated and sulfated metabolites of (-)-epicatechin (EC) and (+)-catechin (Cat) to the protection of human vascular endothelial cells (HUVECs) against oxidative stress. The relative potency of the tested compounds to scavenge superoxide anion radicals showed that a free catechol moiety in the molecule is important for the direct antioxidant activity. EC and Cat (0.5, 1, 10µM) were potent radical scavengers and provided protection against intracellular oxidative stress induced by hydrogen peroxide. Although the metabolites provided less intracellular protection compared to EC and Cat, the tested methylated and glucuronidated metabolites reduced oxidative stress significantly in HUVECs. Our results indicate that the metabolites have a relevant contribution in the intracellular protection of EC and Cat against oxidative stress. Also, the direct antioxidant activity plays an important role in this protection.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/23747595
Author(s): Bast A, Haenen GR
Author(s): Biesalski HK, Grune T, Tinz J, Zöllner I, Blumberg JB
Author(s): Weseler AR, Bast A
Author(s): He HP, Corke H
Author(s): Tinkel J, Hassanain H, Khouri SJ
Author(s): Calabrese V, Cornelius C, Dinkova-Kostova AT, Iavicoli I, Di Paola R, et al.
Author(s): Birringer M
Author(s): Forman HJ, Davies KJ, Ursini F
Author(s): Yelisyeyeva O, Semen K, Zarkovic N, Kaminskyy D, Lutsyk O, et al.
Author(s): Tikekar RV, Ludescher RD, Karwe MV
Author(s): Medina-Franco JL, Giulianotti MA, Welmaker GS, Houghten RA
Author(s): Huerta-Ocampo JA, Barba de la Rosa AP
Author(s): Yelisyeyeva OP, Kamisnkyy DV, Cherkas AP, Ambarova LI, Vyshemyrska OR, et al.
Author(s): Yelisyeyeva OP, Semen KO, Ostrovska GV, Kaminskyy DV, Sirota TV, et al.
Author(s): Yelisyeyeva O, Cherkas A, Semen K, Kaminskyy D, Lutsyk A
Author(s): Sen CK, Khanna S, Roy S
Author(s): Floyd RA, Wiseman BB
Author(s): Sirota TV
Author(s): Peshavariya HM, Dusting GJ, Selemidis S
Author(s): Wang H, Joseph JA
Author(s): Pasko P, Bartn H, Zagrodizki P, Chlopicka J, Izewska A, et al.
Author(s): Martirosyan DM, Miroshnichenko LA, Kulakova SN, Pogojeva AV, Zoloedov VI
Author(s): Aoun M, Feillet-Coudray C, Fouret G, Chabi B, Crouzier D, et al.
Author(s): Chatgilialoglu C, Ferreri C
Author(s): Puca AA, Andrew P, Novelli V, Anselmi CV, Somalvico F, et al.
Author(s): Reynolds CM, Loscher CE, Moloney AP, Roche HM
Author(s): Warleta F, Campos M, Allouche Y, Sánchez-Quesada C, Ruiz-Mora J, et al.
Author(s): Misra HP, Fridovich I
Author(s): Qi S, den Hartog GJ, Bast A
Author(s): Bast A, Weseler AR, Haenen GR, den Hartog GJ