Author(s): Tam JC, Lau KM, Liu CL, To MH, Kwok HF, et al.
Ethnopharmacological relevance: The herbs Radix Astragali (RA) and Radix Rehmanniae (RR) have long been used in traditional Chinese Medicine and serve as the principal herbs in treating diabetic foot ulcer.
Aim of study: Diabetic complications, such as foot ulcer, impose major public health burdens worldwide. In our previous clinical studies, two Chinese medicine formulae F1 and F2 have achieved over 80% limb salvage. A simplified 2-herb formula (NF3) comprising of RA and RR in the ratio of 2:1 was used for further study. NF3 was examined for the ulcer healing effect in diabetic rats, and its potential mechanisms of action in fibroblast proliferation, angiogenesis and anti-inflammation in vitro.
Materials and methods: A chemically induced diabetic foot ulcer rat model was used for studying the wound healing effect. In the in vitro mechanistic studies, human fibroblast cells (Hs27), human umbilical vein endothelial cells (HUVEC) and mouse macrophage cells (RAW264.7) were assessed for tissue regeneration, angiogenesis and anti-inflammatory activities, respectively.
Results: Our in vivo results demonstrated a significant reduction of wound area at day 8 in NF3 (0.98g/kg) group as compared to control (p<0.01). NF3 could significantly stimulate Hs27 proliferation in a dose dependent manner (p<0.05). Besides, NF3 could significantly increase the cell migration and tube formation (p<0.05-0.001) of HUVEC in the angiogenesis study. Furthermore, significant inhibition of nitric oxide production (p<0.01) was found in NF3-treated macrophage cells, suggesting its anti-inflammatory activity.
Conclusions: Our study presents for the first time scientific evidence towards the efficacy of the two-herb formula NF3 in enhancing diabetic wound healing through the actions of tissue regeneration, angiogenesis and anti-inflammation.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/21291991
Author(s): Cheung LW, Leung KW, Wong CK, Wong RN, Wong AS
Author(s): Fan TP, Yeh JC, Leung KW, Yue PY, Wong RN
Author(s): Majewska I, Gendaszewska-Darmach E
Author(s): Tang JY, Li S, Li ZH, Zhang ZJ, Hu G, et al.
Author(s): Hong SJ, Wan JB, Zhang Y, Hu G, Lin HC, et al.
Author(s): Zhang Y, Hu G, Lin HC, Hong SJ, Deng YH, et al.
Author(s): Chung BH, Cho YL, Kim JD, Jo HS, Won MH, et al.
Author(s): Lee TH, Lee GW, Kim CW, Bang MH, Baek NI, et al.
Author(s): Meng H, Guo J, Sun JY, Pei JM, Wang YM, et al.
Author(s): Cárdenas C, Quesada AR, Medina MA
Author(s): Zheng KY, Choi RC, Cheung AW, Guo AJ, Bi CW, et al.
Author(s): Lin CM, Chiu JH, Wu IH, Wang BW, Pan CM, et al.
Author(s): Lee JH, Kim HL, Lee MH, You KE, Kwon BJ, et al.
Author(s): Liao QF, Bi KS
Author(s): Hu G, Long C, Murrell G, Liu K
Author(s): Hwang B, Cho J, Hwang IS, Jin HG, Woo ER, et al.
Author(s): Süntar IP, Akkol EK, Yalçin FN, Koca U, Keles H, et al.
Author(s): Chiou WF, Chen CC, Hung MH
Author(s): Lin CN, Tome WP
Author(s): Shokrzadeh M, SaeediSarvari SS
Author(s): Lin CN, Tome WP
Author(s): Potente M, Gerhardt H, Carmeliet P
Author(s): Singer AJ, Clark RA
Author(s): Lam HW, Lin HC, Lao SC, Gao JL, Hong SJ, et al.
Author(s): Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, et al.