The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity

Author(s): Molyneux P

Abstract

The use of the stable free radical diphenylpicrylhydrazyl (DPPH) to estimate the activity of antioxidants is reviewed. Current applications of the method are examined, particularly the use of the parameter EC50 (substrate concentration to produce 50% reduction of the DPPH). Some recommendations are made as to the most suitable ways of carrying out this assay and evaluating the data produced.

Similar Articles

Phytochemical constituents and antioxidant activity of extract from the leaves of Ocimumgratissimum

Author(s): Alkinmoladum AC, Ibukun EO, Ator E, Obuotor EM, Farombi EO

Phytochemical and antioxidants for health and medicine

Author(s): Krishnaiah D, Sarbatly R, Bono A

Manual of clinical microbiology

Author(s): Lennette EH, Balows A, HauslerJrad WJ, Shadowny HJ

Fatty Acids from Memecylonumbellatum (Burm

Author(s): Joshi H, Joshi AB, Sati H, Gururaja MP, Shetty PR, et al.

Fatty acids isolated from MilletiaversicolorBaker (Fabaceae)

Author(s): Ongoka PR, Banzounzi JT, Poupat C, Ekouya A, Ouamba JM, et al.

Free radical scavenging capacity in the aging of selected red Spanish wines

Author(s): Larrauri JA, Sánchez-Moreno C, Rupérez P, Saura-Calixto F

Free radical scavenging capacity of selected red and white wine

Author(s): Larrauri JA, Sánchez-Moreno C, Saura-Calixto F

Free radical scavenger and antihepatotoxic activity of Rosmarinustomentosus

Author(s): Concepción Navarro M, PilarMontilla M, Martín A, Jiménez J, PilarUtrilla M

Natural antioxidants in grapes and wines

Author(s): Kanner J, Frankel EN, Granit R, German B, Kinsella JE