Wound healing potential of Sambucusebulus L

Author(s): Süntar IP, Akkol EK, Yalçin FN, Koca U, Keles H, et al.


Ethnopharmacological relevance: The leaves of Sambucus ebulus L. are used in Turkish folk medicine for treatment of high fever, rheumatic pains, snake bites and wounds.

Aim of the study: Four different solvent extracts by using n-hexane, diethyl ether, ethyl acetate and methanol were prepared from the leaves of Sambucus ebulus in order to investigate the claimed wound healing activity in vivo and isolation of active component(s) from the active extract through the bioassay-guided fractionation procedures.

Materials and methods: Linear incision and circular excision wound models on rats and mice were employed. The methanol extract was fractionated by successive chromatographic techniques. Wound healing activity of each fraction was investigated following the bioassay-guided fractionation procedures. Moreover, the tissue samples were examined histopathologically.

Results: Significant wound healing activity was observed for the ointment prepared with methanol extract at 1% concentration. The methanol extract treated groups of animals showed 84.3% contraction in circular excision model, which was close to contraction value of the reference drug Madecassol (100%). On the other hand, a significant increase (43.7%) in the wound tensile strength was determined with the same extract on incision wound model. Subfractions showed significant but reduced wound healing activity on both in vivo wound models. A flavonoid derivative "quercetin 3-O-glucoside" was isolated and determined as one of the active component of active final subfraction. The results of histopathological examination supported the outcome of linear incision and circular excision wound models.

Conclusion: The experimental data revealed that the methanolic extract of Sambucus ebulus leaves displayed remarkable wound healing activity.

Similar Articles

Angiogenesis: from plants to blood vessels

Author(s): Fan TP, Yeh JC, Leung KW, Yue PY, Wong RN

Angiogenic effects of the extracts from Chinese herbs: Angelica and Chuanxiong

Author(s): Meng H, Guo J, Sun JY, Pei JM, Wang YM, et al.

Effects of ursolic acid on different steps of the angiogenic process

Author(s): Cárdenas C, Quesada AR, Medina MA

Ferulic acid augments angiogenesis via VEGF, PDGF and HIF-1 alpha

Author(s): Lin CM, Chiu JH, Wu IH, Wang BW, Pan CM, et al.

Basic and therapeutic aspects of angiogenesis

Author(s): Potente M, Gerhardt H, Carmeliet P

Cutaneous wound healing

Author(s): Singer AJ, Clark RA

Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts

Author(s): Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, et al.