Author(s): Koneri K, Goi T, Hirono Y, Katayama K, Yamaguchi A
Background:The Beclin 1 gene binds to the apoptosis-inhibiting protein Bcl-2. The expression and function of the Beclin 1 gene in four colorectal cancer cell lines (HT29, DLD-1, SW480, SW620) was investigated.
Materials and methods:The expression of Beclin 1 mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR), the cell growth rate of a stable cell line established after transfection of the Beclin 1 gene into low Beclin 1-expressing HT29 colon cancer cells. In addition, changes in cell cycle were investigated by flow cytometry, and the expression levels of cell cycle related proteins in the Beclin 1 transfected colon cancer cell line were assessed.
Results:The expression level of Beclin 1 mRNA in the colon cancer cell lines was variable. Transfection of the low Beclin 1 gene-expressing colon cancer cell line with the Beclin 1 gene resulted in cell growth inhibition. The cell cycle analysis showed that the percentage of GJ-phase cells was significantly higher in Beclin 1 transfectants than in mock transfected cells. The expression levels of cyclin E and phosphorylated Rb were decreased.
Conclusion:These results indicate that Beclin 1 can inhibit the growth of colorectal cancer cells.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/17595761
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