Author(s): Schalper K, Velcheti V, Carvajal D, Wimberly H, Brown J, et al.
Purpose: Blockade of the PD-1/PD-L1 axis emerged as a promising new therapeutic option for cancer that has resulted in lasting responses in metastatic renal, lung carcinomas, and melanomas. Tumor PD-L1 protein expression may predict response to drugs targeting this pathway. Measurement of PD-L1 protein is limited by the lack of standardized immunohistochemical methods and variable performance of antibodies. Our goal was to correlate PD-L1 mRNA expression with clinical variables in primary breast carcinomas.
Experimental Design: The fluorescent RNAscope paired-primer assay was used to quantify in situ PD-L1 mRNA levels in 636 stage I–III breast carcinomas on two sets of tissue microarrays [YTMA128 (n = 238) and YTMA201 (n = 398)]. Tumor-infiltrating lymphocytes (TIL) were assessed by hematoxylin/eosin stain and quantitative fluorescence.
Referred From: https://aacrjournals.org/clincancerres/article/20/10/2773/78530/In-Situ-Tumor-PD-L1-mRNA-Expression-Is-Associated
Author(s): Aptsiauri N, Cabrera T, Mendez R, Garcia-Lor A, Ruiz-Cabello F, et al.
Author(s): Shenoy SK, Drake MT, Nelson CD, Houtz DA, Xiao K, et al.
Author(s): Boon T, Coulie PG, Van Den Eynde BJ, Van Der Bruggen P
Author(s): Van Raamsdonk C, Griewank K, Michelle B, Crosby MB, Garrido MC, et al.
Author(s): Remeseiro S, Cuadrado A, Carretero M, Martínez P, Drosopoulos WC, et al.
Author(s): Cheng AL, Kang YK, Chen Z, Tsao CJ, Qin S, et al.
Author(s): Paschen A, Arens N, Sucker A, Greulich-Bode KM, Fonsatti E, et al.
Author(s): Gembarska A, Luciani F, Fedele C, Russell EA, Dewaele A, et al.
Author(s): Iwatsuki M, Mimori K, Yokobori T, Ishi H, Beppu T, et al.
Author(s): Kokkola R, Andersson A, Mullins G, Östberg T, Treutiger CJ, et al.
Author(s): Parry RV, Chemnitz JM, Frauwirth KA, Lanfranco AR, Braunstein I, et al.
Author(s): Iwai Y, Ishida M, Tanaka Y, Okazaki T, Honjo T, et al.
Author(s): Mani A, Guo W, Liao MJ, Eaton E, Ayyanan A, et al.
Author(s): Azuma T, Yao S, Zhu G, Flies A, Flies S, et al.
Author(s): Gradishar W, Anderson B, Blair S, Burstein H, Cyr A, et al.
Author(s): Ghebeh H, Barhoush E, Tulbah A, Elkum N, Al-Tweigeri T, et al.
Author(s): Muenst S, Schaerli AR, Gao F, Däster S, Trella E, et al.
Author(s): Twyman-Saint VC, Rech AJ, Maity A, Rengan R, Pauken KE, et al.
Author(s): Taube JM, Klein A, Brahmer JR, Xu H, Pan X, et al.
Author(s): Chow E, Danielewski JA, Fehler G, Tabrizi SN, Plaw MG, et al.
Author(s): Cooke NM, Spillane CD, Sheils O, O’Leary J, Kenny D
Author(s): Kitamura T, Qian BZ, Pollard JW
Author(s): Kobayashi N, Hiraoka N, Yamagami W, Ojima H, Kanai Y, et al.
Author(s): Dworkin AM, Spearman A, Tseng S, Sweet K, Amanda AE
Author(s): Thiery JP, Acloque H, Huang R, Nieto A
Author(s): Bastian P, Yegnasubramanian S, Palapattu G, Rogers C, Lin X, et al.
Author(s): Nagarajan R, Costello J
