Author(s): Mani A, Guo W, Liao MJ, Eaton E, Ayyanan A, et al.
The epithelial-mesenchymal transition (EMT) is a key developmental program that is often activated during cancer invasion and metastasis. We here report that the induction of an EMT in immortalized human mammary epithelial cells (HMLEs) results in the acquisition of mesenchymal traits and in the expression of stem-cell markers. Furthermore, we show that those cells have an increased ability to form mammospheres, a property associated with mammary epithelial stem cells. Independent of this, stem cell-like cells isolated from HMLE cultures form mammospheres and express markers similar to those of HMLEs that have undergone an EMT. Moreover, stem-like cells isolated either from mouse or human mammary glands or mammary carcinomas express EMT markers. Finally, transformed human mammary epithelial cells that have undergone an EMT form mammospheres, soft agar colonies, and tumors more efficiently. These findings illustrate a direct link between the EMT and the gain of epithelial stem cell properties.
Author(s): Aptsiauri N, Cabrera T, Mendez R, Garcia-Lor A, Ruiz-Cabello F, et al.
Author(s): Shenoy SK, Drake MT, Nelson CD, Houtz DA, Xiao K, et al.
Author(s): Boon T, Coulie PG, Van Den Eynde BJ, Van Der Bruggen P
Author(s): Van Raamsdonk C, Griewank K, Michelle B, Crosby MB, Garrido MC, et al.
Author(s): Remeseiro S, Cuadrado A, Carretero M, Martínez P, Drosopoulos WC, et al.
Author(s): Cheng AL, Kang YK, Chen Z, Tsao CJ, Qin S, et al.
Author(s): Paschen A, Arens N, Sucker A, Greulich-Bode KM, Fonsatti E, et al.
Author(s): Gembarska A, Luciani F, Fedele C, Russell EA, Dewaele A, et al.
Author(s): Iwatsuki M, Mimori K, Yokobori T, Ishi H, Beppu T, et al.
Author(s): Kokkola R, Andersson A, Mullins G, Östberg T, Treutiger CJ, et al.
Author(s): Parry RV, Chemnitz JM, Frauwirth KA, Lanfranco AR, Braunstein I, et al.
Author(s): Iwai Y, Ishida M, Tanaka Y, Okazaki T, Honjo T, et al.
Author(s): Azuma T, Yao S, Zhu G, Flies A, Flies S, et al.
Author(s): Gradishar W, Anderson B, Blair S, Burstein H, Cyr A, et al.
Author(s): Schalper K, Velcheti V, Carvajal D, Wimberly H, Brown J, et al.
Author(s): Ghebeh H, Barhoush E, Tulbah A, Elkum N, Al-Tweigeri T, et al.
Author(s): Muenst S, Schaerli AR, Gao F, Däster S, Trella E, et al.
Author(s): Twyman-Saint VC, Rech AJ, Maity A, Rengan R, Pauken KE, et al.
Author(s): Taube JM, Klein A, Brahmer JR, Xu H, Pan X, et al.
Author(s): Chow E, Danielewski JA, Fehler G, Tabrizi SN, Plaw MG, et al.
Author(s): Cooke NM, Spillane CD, Sheils O, O’Leary J, Kenny D
Author(s): Kitamura T, Qian BZ, Pollard JW
Author(s): Kobayashi N, Hiraoka N, Yamagami W, Ojima H, Kanai Y, et al.
Author(s): Dworkin AM, Spearman A, Tseng S, Sweet K, Amanda AE
Author(s): Thiery JP, Acloque H, Huang R, Nieto A
Author(s): Bastian P, Yegnasubramanian S, Palapattu G, Rogers C, Lin X, et al.
Author(s): Nagarajan R, Costello J