Author(s): Zhou XW, Fan ZQ, Chen Y, Zhu YL, Li JY, et al.
The flavonoids metabolic pathway plays central roles in floral coloration, in which anthocyanins and flavonols are derived from common precursors, dihydroflavonols. Flavonol synthase (FLS) catalyses dihydroflavonols into flavonols, which presents a key branch of anthocyanins biosynthesis. The yellow flower of Camellia nitidissima Chi. is a unique feature within the genus Camellia, which makes it a precious resource for breeding yellow camellia varieties. In this work, we characterized the secondary metabolites of pigments during floral development of C. nitidissima and revealed that accumulation of flavonols correlates with floral coloration. We first isolated CnFLS1 and showed that it is a FLS of C. nitidissima by gene family analysis. Second, expression analysis during floral development and different floral organs indicated that the expression level of CnFLS1 was regulated by developmental cues, which was in agreement with the accumulating pattern of flavonols. Furthermore, over-expression of CnFLS1 in Nicotiana tabacum altered floral colour into white or light yellow, and metabolic analysis showed significant increasing of flavonols and reducing of anthocyanins in transgenic plants. Our work suggested CnFLS1 plays critical roles in yellow colour pigmentation and is potentially a key point of genetic engineering toward colour modification in Camellia.
Referred From: https://pubmed.ncbi.nlm.nih.gov/23938391/
Author(s): Ran Y, Simpson S
Author(s): Chandler SF, Brugliera F
Author(s): Ferrer JL, Austin MB, Stewart C Jr, Noel JP
Author(s): Winkel-Shirley B
Author(s): Buer CS, Imin N, Djordjevic MA
Author(s): Falcone Ferreyra ML, Rius SP, Casati P
Author(s): Clegg MT, Durbin ML
Author(s): Kim S, Binzel ML, Yoo KS, Park S, Pike LM
Author(s): Samappito S, Page J, Schmidt J, De-Eknamkul W, Kutchan TM
Author(s): Ferrer JL, Jez JM, Bowman ME, Dixon RA, Noel JP
Author(s): Jez JM, Ferrer JL, Bowman ME, Austin MB, Schröder J, et al.