Recommended Conferences

Genetic Engineering and Gene Therapy

Paris, France

Human Genetics and Genetic Disorders

Miami, USA

Tissue Engineering and Regenerative Medicine

Chicago, USA
Related Subjects
 

Pink (P), a new locus responsible for a pink trait in onions (Allium cepa) resulting from natural mutations of anthocyanidin synthase

Author(s): Kim S, Binzel ML, Yoo KS, Park S, Pike LM

Abstract

A new locus conditioning a pink trait in onions was identified. Unusual pink onions were found in haploid populations induced from an F1 hybrid between yellow and dark red parents and in F3 populations originating from the same cross. Segregation ratios of red to pink in F2, backcross, and F3 populations indicated that this pink trait is determined by a single recessive locus. RT-PCR was carried out to look for any differential expression of anthocyanin synthesis genes between dark red and pink F3 lines. The transcript level of anthocyanidin synthase (ANS) was significantly reduced in the pink line. To determine whether this reduced transcription is caused by other regulatory factors or by mutations in the ANS gene itself, ANS gene sequences from both dark red and pink F3 lines were compared to detect any polymorphisms. Polymorphisms were identified, and subsequently utilized as molecular markers for the selection of ANS alleles. Absolute co-segregation of the pink allele and the ANS allele from the pink line was observed in parents, F1 and F3 populations. These results indicate that reduced transcription of the ANS gene caused by mutations in a cis -acting element is likely to result in the pink trait in onions.

Similar Articles

Genetic modification in floriculture

Author(s): Chandler SF, Brugliera F

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids

Author(s): Ferrer JL, Austin MB, Stewart C Jr, Noel JP

Flavonoids: new roles for old molecules

Author(s): Buer CS, Imin N, Djordjevic MA

Molecular characterization of root-specific chalcone synthases from Cassia alata

Author(s): Samappito S, Page J, Schmidt J, De-Eknamkul W, Kutchan TM

Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis

Author(s): Ferrer JL, Jez JM, Bowman ME, Dixon RA, Noel JP

Structure and mechanism of chalcone synthase-like polyketide synthases

Author(s): Jez JM, Ferrer JL, Bowman ME, Austin MB, Schröder J, et al.