Recommended Conferences

Human Genetics and Genetic Disorders

Miami, USA

Tissue Engineering and Regenerative Medicine

Chicago, USA
Related Subjects
 

RNA-Seq analysis of a soybean near-isogenic line carrying bacterial leaf pustule-resistant and-susceptible alleles

Author(s): Kim KH, Kang YJ, Kim DH, Yoon MY, Moon J-K, et al.

Abstract

Bacterial leaf pustule (BLP) disease is caused by Xanthomonas axonopodis pv. glycines (Xag). To investigate the plant basal defence mechanisms induced in response to Xag, differential gene expression in near-isogenic lines (NILs) of BLP-susceptible and BLP-resistant soybean was analysed by RNA-Seq. Of a total of 46 367 genes that were mapped to soybean genome reference sequences, 1978 and 783 genes were found to be up- and down-regulated, respectively, in the BLP-resistant NIL relative to the BLP-susceptible NIL at 0, 6, and 12h after inoculation (hai). Clustering analysis revealed that these genes could be grouped into 10 clusters with different expression patterns. Functional annotation based on gene ontology (GO) categories was carried out. Among the putative soybean defence response genes identified (GO:0006952), 134 exhibited significant differences in expression between the BLP-resistant and -susceptible NILs. In particular, pathogen-associated molecular pattern (PAMP) and damage-associated molecular pattern (DAMP) receptors and the genes induced by these receptors were highly expressed at 0 hai in the BLP-resistant NIL. Additionally, pathogenesis-related (PR)-1 and -14 were highly expressed at 0 hai, and PR-3, -6, and -12 were highly expressed at 12 hai. There were also significant differences in the expression of the core JA-signalling components MYC2 and JASMONATE ZIM-motif. These results indicate that powerful basal defence mechanisms involved in the recognition of PAMPs or DAMPs and a high level of accumulation of defence-related gene products may contribute to BLP resistance in soybean.

Similar Articles

Effect of climatic factors on powdery mildew caused by Sphaerotheca macularis f

Author(s): Amsalem L, Freeman S, Rav-David D, Nitzani Y, Sztejnberg A, et al.

Biotechnological concepts for improving plant innate immunity

Author(s): Gust AA, Brunner F, Nurnberger T

The genome of woodland strawberry (Fragaria vesca)

Author(s): Shulaev V, Sargent DJ, Crowhurst RN, Mockler TC, Folkerts O, et al.

An inbred line of the diploid strawberry Fragaria vesca f

Author(s): Slovin JP, Schmitt K, Folta KM

Cytochromes P450 for engineering herbicide tolerance

Author(s): Werck-Reichhart D, Hehn A, Didierjean L

The barley/Blumeria (syn

Author(s): Thordal-Christensen H, Gregersen PL, Collinge DB

The HvNAC6 transcription factor: a positive regulator of penetration resistance in barley and Arabidopsis

Author(s): Jensen MK, Rung JH, Gregersen PL, Gjetting T, Fuglsang AT, et al.